Cryopreservation

Conservation of dispended cells and 3D tissues

We have developed individual cryo-protocols which enable us to freeze adherent keratinocytes or fi broblasts as they leave the cell expansion module. These cell specific protocols minimize washing and handling steps. Usage of toxic substances is no longer necessary, hence minimizing detrimental effects of cryo-protective media. Currently, our team is working on a fully automated low temperature handling device which can implement the procedures specifi ed in the cryo-protocols and store the frozen adherent keratinocytes and fi broblasts. The main challenge in matching the protocols consists in complying with the required heating and freezing rates. As adherent cells are in direct contact with a membrane which in turn is surrounded by the bioreactor, controlled heat transfer via nitrogen is one of the major challenges. Once the fully automated freezing and thawing of adherent cells has been demonstrated, the technology can be adapted to skin equivalents in 24-well plates.

Specifications

  • Temporary storage of keratinocytes and fi broblasts
  • Flexible freezing protocols down to -130°C for keratinocytes and fibroblasts
  • Thawing protocols for keratinocytes and fibroblasts
  • Single sample tracking